Anti-MeCP2 antibody - ChIP Grade (ab195393)
Key features and details
- Rabbit polyclonal to MeCP2 - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MeCP2 antibody - ChIP Grade
See all MeCP2 primary antibodies -
Description
Rabbit polyclonal to MeCP2 - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human MeCP2 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P51608 -
Positive control
- HeLa cell nuclear extract; chromatin from human osteosarcoma (U2OS) cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-MeCP2 antibody - ChIP Grade (ab195393)
ChIP assays were performed using human osteosarcoma (U2OS) cells, ab195393 and optimized PCR primer sets. Sheared chromatin from 1x106 cells and 5 μg of antibody were used per ChIP experiment. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). Image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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Western blot - Anti-MeCP2 antibody - ChIP Grade (ab195393)Anti-MeCP2 antibody - ChIP Grade (ab195393) at 1/1000 dilution + nuclear extract from HeLa cells at 40 µgTBS-Tween containing 5% skimmed milk
