Anti-MeCP2 antibody [5H12] (ab277629)
![Anti-MeCP2 antibody [5H12] (ab277629)](https://www.abcam.com/ps/products/277/ab277629/Images/ab277629-438738-AntiMeCP2-antibody-5H12-IHC-rat-cerebellum.JPG "Anti-MeCP2 antibody [5H12] (ab277629)")
Key features and details
- Mouse monoclonal [5H12] to MeCP2
- Suitable for: IHC, WB
- Reacts with: Mouse, Rat, Cow
- Isotype: IgG2b
Overview
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Product name
Anti-MeCP2 antibody [5H12]
See all MeCP2 primary antibodies -
Description
Mouse monoclonal [5H12] to MeCP2 -
Host species
Mouse -
Tested applications
Suitable for: IHC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow -
Immunogen
Recombinant full length protein corresponding to Human MeCP2.
Database link: P51608 -
Positive control
- WB: Nuclear extract of rat brain, nuclear extract of mouse brain and cow cerebellum. IHC: Rat cerebellum.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from Tissue Culture supernatant. -
Clonality
Monoclonal -
Clone number
5H12 -
Isotype
IgG2b -
Research areas
Images
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Immunohistochemistry - Anti-MeCP2 antibody [5H12] (ab277629)
Perfusion-fixed, free floating sections of rat cerebellum section stained for MeCP2 using ab277629 (red) at 1/1000 dilution, and counterstained with rabbit pAb to GFAP (green) at 1/5000 dilution in immunohistochemical analysis. The blue Hoechst is staining nuclear DNA. ab277629 specifically labels nuclei of neurons, while the GFAP antibody stains the network of astroglial cells and projections of Bergmann glia.
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![Western blot - Anti-MeCP2 antibody [5H12] (ab277629)](https://www.abcam.com/ps/products/277/ab277629/Images/ab277629-438737-AntiMeCP2-antibody-5H12-WB-nuclear-extract-of-rat-brain-nuclear-extract-of-mouse-brain-cow-cerebellum.JPG)
Western blot - Anti-MeCP2 antibody [5H12] (ab277629)Lanes 2-4 : Anti-MeCP2 antibody [5H12] (ab277629) at 1/2000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : nuclear extract of rat brain
Lane 3 : nuclear extract of mouse brain
Lane 4 : cow cerebellum
Predicted band size: 52 kDa