Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ME3 antibody [EPR10378] (ab172972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10378] to ME3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ME3 antibody [EPR10378]
    See all ME3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10378] to ME3

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ME3 aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16798

  • Positive control

    • Fetal brain, Fetal kidney, Fetal heart and HeLa lysates. paraffin-embedded Human brain tissue and Human thyroid tissue. Mouse heart, rat heart, rat brain and mouse brain lysates; human colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ME3 antibody [EPR10378] (ab172972)
    Western blot - Anti-ME3 antibody [EPR10378] (ab172972)
    All lanes : Anti-ME3 antibody [EPR10378] (ab172972) at 1/5000 dilution (purified)

    Lane 1 : Human fetal kidney lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Rat heart lysates
    Lane 4 : Mouse brain lysates
    Lane 5 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling ME3 with Purified ab172972 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-ME3 antibody [EPR10378] (ab172972)
    Western blot - Anti-ME3 antibody [EPR10378] (ab172972)
    All lanes : Anti-ME3 antibody [EPR10378] (ab172972) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal brain lysate
    Lane 2 : Fetal kidney lysate
    Lane 3 : Fetal heart lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 67 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ME3 with unpurified ab172972 at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (ab172972)

    Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling ME3 with unpurified ab172972 at 1/50 dilution.

  • Anti-ME3 antibody [EPR10378] (ab172972)
    Anti-ME3 antibody [EPR10378] (ab172972)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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