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Anti-MDC1 antibody [P2B11] (ab241048)

Key features and details

  • Mouse monoclonal [P2B11] to MDC1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-MDC1 antibody [P2B11]
    See all MDC1 primary antibodies

  • Description

    Mouse monoclonal [P2B11] to MDC1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Mouse MDC1. (From around the N-terminal of NP_001010833.2)
    Database link: Q5PSV9

  • Positive control

    • ICC/IF: NIH/3T3 cells. WB: Human 293Trap cell lysates. Mouse cortex and cerebellum lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    P2B11

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MDC1 antibody [P2B11] (ab241048)
    Immunocytochemistry/ Immunofluorescence - Anti-MDC1 antibody [P2B11] (ab241048)

    Immunocytochemistry/Immunofluorescence analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling MDC1 (Green) using ab241048 at 1:100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.

    Secondary antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 minutes at RT.

    Counterstain: DAPI (blue) nuclear stain at 1:5000, 5 minutes RT.

    (A) DAPI (blue) nuclear stain (B) ab241048 (C) Composite.

  • Western blot - Anti-MDC1 antibody [P2B11] (ab241048)
    Western blot - Anti-MDC1 antibody [P2B11] (ab241048)
    All lanes : Anti-MDC1 antibody [P2B11] (ab241048) at 1/1000 dilution (2 hours at RT)

    Lane 1 : Mouse cortex lysate
    Lane 2 : Mouse cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse HRP-IgG for 60 minutes at RT at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Block: 5% Skimmed milk in 1X TBST

  • Western blot - Anti-MDC1 antibody [P2B11] (ab241048)
    Western blot - Anti-MDC1 antibody [P2B11] (ab241048)
    Anti-MDC1 antibody [P2B11] (ab241048) at 1/1000 dilution (2 hours at RT) + Human 293Trap cell lysates at 30 µg

    Secondary
    Goat anti-Mouse HRP-IgG for 60 minutes at RT at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


     Block: 5% Skimmed milk in 1X TBST.

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