Anti-MCU antibody [CL3576] (ab219827)
Key features and details
- Mouse monoclonal [CL3576] to MCU
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MCU antibody [CL3576] -
Description
Mouse monoclonal [CL3576] to MCU -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human MCU aa 250-350.
Database link: Q8NE86 -
Epitope
Binds to an epitope located within the peptide sequence RDRQYLLFFH as determined by overlapping synthetic peptides. -
Positive control
- WB: A549 cell extracts; ICC: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL3576 -
Isotype
IgG1 -
Research areas
Images
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PFA-fixed, Triton X-100 permeabilized A549 cells stained for MCU (Green) using ab219827 at 4 μg/ml in ICC/IF.
Microtublues are stained red, nuclei are stained blue.
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All lanes : Anti-MCU antibody [CL3576] (ab219827) at 1/500 dilution
Lane 1 : A549 transfected with control siRNA cell extract
Lane 2 : A549 transfected with target specific siRNA #1 cell extract
Lane 3 : A549 transfected with target specific siRNA #2 cell extract
Predicted band size: 40 kDaDownregulation of antibody signal confirms target specificity. Remaining % intensity, relative to the control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.