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Cardiovascular Heart Cardiac metabolism

Anti-MCU antibody [CL3576] (ab219827)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-MCU antibody [CL3576] (ab219827)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [CL3576] to MCU
  • Suitable for: ICC, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-MCU antibody [CL3576]
  • Description

    Mouse monoclonal [CL3576] to MCU
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human MCU aa 250-350.
    Database link: Q8NE86

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope

    Binds to an epitope located within the peptide sequence RDRQYLLFFH as determined by overlapping synthetic peptides.
  • Positive control

    • WB: A549 cell extracts; ICC: A549 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    CL3576
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Images

  • Immunocytochemistry - Anti-MCU antibody [CL3576] (ab219827)
    Immunocytochemistry - Anti-MCU antibody [CL3576] (ab219827)

    PFA-fixed, Triton X-100 permeabilized A549 cells stained for MCU (Green) using ab219827 at 4 μg/ml in ICC/IF.

    Microtublues are stained red, nuclei are stained blue.

  • Western blot - Anti-MCU antibody [CL3576] (ab219827)
    Western blot - Anti-MCU antibody [CL3576] (ab219827)
    All lanes : Anti-MCU antibody [CL3576] (ab219827) at 1/500 dilution

    Lane 1 : A549 transfected with control siRNA cell extract
    Lane 2 : A549 transfected with target specific siRNA #1 cell extract
    Lane 3 : A549 transfected with target specific siRNA #2 cell extract

    Predicted band size: 40 kDa



    Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative to the control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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