Anti-TNNI3K antibody (ab136954)
Key features and details
- Rabbit polyclonal to TNNI3K
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TNNI3K antibody
See all TNNI3K primary antibodies -
Description
Rabbit polyclonal to TNNI3K -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human TNNI3K aa 300-400 conjugated to keyhole limpet haemocyanin.
Database link: Q59H18 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Fetal Heart; Mouse Heart; Rat Heart.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatHumanAll applications RabbitChimpanzeeMacaque monkeyApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).Target
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Function
May play a role in cardiac physiology. -
Tissue specificity
Highly expressed in both adult and fetal heart. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 10 ANK repeats.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Nucleus. Cytoplasm. Expressed at lower levels in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 100526835 Human
- Entrez Gene: 51086 Human
- Entrez Gene: 435766 Mouse
- Entrez Gene: 295531 Rat
- SwissProt: Q59H18 Human
- SwissProt: Q5GIG6 Mouse
- SwissProt: Q7TQP6 Rat
- Unigene: 729320 Human
see all -
Alternative names
- Cardiac ankyrin repeat kinase antibody
- cardiac muscle antibody
- Cardiac troponin I-interacting kinase antibody
see all
Images
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Western blot - Anti-TNNI3K antibody (ab136954)All lanes : Anti-TNNI3K antibody (ab136954) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136954 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
References (0)
ab136954 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-TNNI3K antibody (ab136954)All lanes : Anti-TNNI3K antibody (ab136954) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136954 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
