Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCK10/NEP antibody [EPR22316-508] - BSA and Azide free (ab261728)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22316-508] to MCK10/NEP - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-MCK10/NEP antibody [EPR22316-508] - BSA and Azide free
    See all MCK10/NEP primary antibodies

  • Description

    Rabbit monoclonal [EPR22316-508] to MCK10/NEP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MCF7 and T-47D cells. Flow Cyt: HeLa cells.

  • General notes

    ab261728 is the carrier-free version of ab255810.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MCK10/NEP antibody [EPR22316-508] - BSA and Azide free (ab261728)
    Immunocytochemistry/ Immunofluorescence - Anti-MCK10/NEP antibody [EPR22316-508] - BSA and Azide free (ab261728)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling MCK10/NEP with ab255810 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in MCF7 cell line. Negative control: HeLa (PMID: 19787223, 24740739) is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Ab255810 anti- MCK10 NEP ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 2 ug/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255810).

  • Flow Cytometry - Anti-MCK10/NEP antibody [EPR22316-508] - BSA and Azide free (ab261728)
    Flow Cytometry - Anti-MCK10/NEP antibody [EPR22316-508] - BSA and Azide free (ab261728)

    Flow cytometric analysis of  HeLa (human cervix adenocarcinoma epithelial cell, Left) / T-47D (human ductal breast epithelial tumor epithelial cell, Right) cells labelling MCK10/NEP with ab255810 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255810).

  • Immunocytochemistry/ Immunofluorescence - Anti-MCK10/NEP antibody [EPR22316-508] - BSA and Azide free (ab261728)
    Immunocytochemistry/ Immunofluorescence - Anti-MCK10/NEP antibody [EPR22316-508] - BSA and Azide free (ab261728)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling MCK10/NEP with ab255810 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in T-47D cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000  dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255810).

  • Anti-MCK10/NEP antibody [EPR22316-508] - BSA and Azide free (ab261728)
    Anti-MCK10/NEP antibody [EPR22316-508] - BSA and Azide free (ab261728)

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