Anti-Major Basic Protein antibody (ab187523)
Key features and details
- Rabbit polyclonal to Major Basic Protein
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Major Basic Protein antibody
See all Major Basic Protein primary antibodies -
Description
Rabbit polyclonal to Major Basic Protein -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant full length protein corresponding to Human Major Basic Protein aa 1 to the C-terminus.
Database link: P13727
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Major Basic Protein antibody (ab187523)All lanes : Anti-Major Basic Protein antibody (ab187523) at 1/1000 dilution
Lane 1 : L-02 (human adenocarcinoma cell line) cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 5 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 6 : Mouse liver tissue lysate
Lane 7 : Mouse spleen tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Diluting buffer: 3% NFDM/TBST
