Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MAGMAS antibody [EPR15214] (ab184157)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15214] to MAGMAS
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MAGMAS antibody [EPR15214]
    See all MAGMAS primary antibodies

  • Description

    Rabbit monoclonal [EPR15214] to MAGMAS

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, LnCaP and Jurkat cell lysates; Human skeletal muscle lysate; Human kidney tissue; mouse skeletal muscle tissue; HepG2 cells.

  • General notes

     

     

     This product was previously labelled as PAM16

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15214

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MAGMAS antibody [EPR15214] (ab184157)
    Western blot - Anti-MAGMAS antibody [EPR15214] (ab184157)
    All lanes : Anti-MAGMAS antibody [EPR15214] (ab184157) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : LnCaP cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-MAGMAS antibody [EPR15214] (ab184157)
    Western blot - Anti-MAGMAS antibody [EPR15214] (ab184157)
    Anti-MAGMAS antibody [EPR15214] (ab184157) at 1/20000 dilution + Human skeletal muscle lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 13 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (ab184157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (ab184157)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MAGMAS with ab184157 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (ab184157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (ab184157)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling MAGMAS with ab184157 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MAGMAS antibody [EPR15214] (ab184157)
    Immunocytochemistry/ Immunofluorescence - Anti-MAGMAS antibody [EPR15214] (ab184157)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling MAGMAS with ab184157 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).

  • Anti-MAGMAS antibody [EPR15214] (ab184157)
    Anti-MAGMAS antibody [EPR15214] (ab184157)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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