Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MAGMAS antibody [EPR15214] (ab184157)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15214] to MAGMAS
Suitable for: ICC/IF, IHC-P, WB
Reacts with: Mouse, Human

Product name

Anti-MAGMAS antibody [EPR15214]
See all MAGMAS primary antibodies
Description

Rabbit monoclonal [EPR15214] to MAGMAS
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HepG2, LnCaP and Jurkat cell lysates; Human skeletal muscle lysate; Human kidney tissue; mouse skeletal muscle tissue; HepG2 cells.
General notes
This product was previously labelled as PAM16

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15214
Isotype

IgG
Research areas

Western blot - Anti-MAGMAS antibody [EPR15214] (ab184157)

All lanes : Anti-MAGMAS antibody [EPR15214] (ab184157) at 1/20000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 14 kDa
Observed band size: 13 kDa
why is the actual band size different from the predicted?
Western blot - Anti-MAGMAS antibody [EPR15214] (ab184157)

Anti-MAGMAS antibody [EPR15214] (ab184157) at 1/20000 dilution + Human skeletal muscle lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 14 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (ab184157)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MAGMAS with ab184157 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (ab184157)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling MAGMAS with ab184157 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MAGMAS antibody [EPR15214] (ab184157)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling MAGMAS with ab184157 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).
Anti-MAGMAS antibody [EPR15214] (ab184157)

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