Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit recombinant multiclonal [RM1008] to LYVE1
Suitable for: Flow Cyt, IHC-Fr, IHC-P, WB, IP
Reacts with: Mouse, Human

Product name

Anti-LYVE1 antibody [RM1008]
See all LYVE1 primary antibodies
Description

Rabbit recombinant multiclonal [RM1008] to LYVE1
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IHC-Fr, IHC-P, WB, IPmore details
Unsuitable for: ICC
Species reactivity

Reacts with: Mouse, Human
Immunogen

This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse lung, A549 lysates. IHC-P: Mouse spleen, liver tissues. IHC-Fr: Mouse lung tissue. Flow Cyt: bEnd.3 cells. IP: Mouse lung lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Recombinant Multiclonal
Clone number

RM1008
Isotype

IgG

Western blot - Anti-LYVE1 antibody [RM1008] (ab281587)

All lanes : Anti-LYVE1 antibody [RM1008] (ab281587) at 1/1000 dilution

Lane 1 : Mouse lung lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat lung lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 35 kDa
Observed band size: 50-70 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Several bands are observed including soluble, glycosylated and non-glycosylated forms which are consistent with the literature (PMID: 26966180).

Exposure time: 37 seconds
Western blot - Anti-LYVE1 antibody [RM1008] (ab281587)

Anti-LYVE1 antibody [RM1008] (ab281587) at 1/1000 dilution + A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution

Predicted band size: 35 kDa
Observed band size: 50-70 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 3 min.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (ab281587)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling LYVE1 with ab281587 at 1/2000 dilution (0.293 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial vessels of mouse spleen. The section was incubated with ab281587 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [RM1008] (ab281587)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling LYVE1 with ab281587 at 1/2000 dilution (0.293 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood sinusoids of mouse liver. The section was incubated with ab281587 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [RM1008] (ab281587)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung tissue labeling LYVE1 with ab281587 at 1/2000 dilution (0.228 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).
Flow Cytometry - Anti-LYVE1 antibody [RM1008] (ab281587)

Flow cytometric analysis of bEnd.3 (mouse brain endothelioma) cells labelling LYVE1 with 281587 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730)/Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Partial LYVE1 expression on bEnd.3 cells is reported in literature (PMID: 25282873).
Immunoprecipitation - Anti-LYVE1 antibody [RM1008] (ab281587)

LYVE1 was immunoprecipitated from 0.35 mg Mouse lung lysate with ab281587 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281587 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse lung lysate 10ug

Lane 2: ab281587 IP in Mouse lung lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab281587 in Mouse lung lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Anti-LYVE1 antibody [RM1008] (ab281587)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LYVE1 antibody [RM1008] (ab281587)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Images