Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7921] to LysRS - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LysRS antibody [EPR7921] - BSA and Azide free
    See all LysRS primary antibodies

  • Description

    Rabbit monoclonal [EPR7921] to LysRS - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248286 is the carrier-free version of ab129080. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248286 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
    Western blot - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
    All lanes : Anti-LysRS antibody [EPR7921] (ab129080) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 68 kDa



    This data was developed using ab129080, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
    Immunocytochemistry - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
    This data was developed using ab129080, the same antibody clone in a different buffer formulation.ICC/IF image of ab129080 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129080 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
    This data was developed using ab129080, the same antibody clone in a different buffer formulation.ab129080, at a dilution of 1/50, staining LysRS in paraffin embedded Human colon tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
    OI-RD Scanning - Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
    This data was developed using ab129080, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)
    Anti-LysRS antibody [EPR7921] - BSA and Azide free (ab248286)

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