Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LYPD3 antibody [EPR9107] (ab151709)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9107] to LYPD3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-LYPD3 antibody [EPR9107]
    See all LYPD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9107] to LYPD3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human LYPD3. The exact sequence is proprietary.

  • Positive control

    • BxPC3, HACAT cell lysates, Human skin tissue, Human esophagus lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9107

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)
    Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)
    All lanes : Anti-LYPD3 antibody [EPR9107] (ab151709) at 1/1000 dilution (purified)

    Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysates
    Lane 2 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : Human esophagus lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000 dilution

    Predicted band size: 36 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue sections labeling LYPD3 with Purified ab151709 at 1:1000 dilution (1.01 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] (ab151709)
    Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] (ab151709)

    ab151709 (purified) at 1:50 dilution (2ug) immunoprecipitating LYPD3 in HaCaT (Human skin keratinocyte) whole cell lysate.
    Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10ug
    Lane 2 (+): ab151709 & HaCaT (Human skin keratinocyte) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151709 in HaCaT (Human skin keratinocyte) whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)
    Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)
    All lanes : Anti-LYPD3 antibody [EPR9107] (ab151709) at 1/1000 dilution (unpurified)

    Lane 1 : BxPC3 cell lysate
    Lane 2 : HACAT cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling LYPD3 with unpurified ab151709 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-LYPD3 antibody [EPR9107] (ab151709)
    Anti-LYPD3 antibody [EPR9107] (ab151709)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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