Anti-LTBP1 antibody (ab78294)
Key features and details
- Rabbit polyclonal to LTBP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-LTBP1 antibody -
Description
Rabbit polyclonal to LTBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human
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Immunogen
Synthetic peptide corresponding to Human LTBP1 aa 1350-1450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87128) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Rat Liver; Mouse Pancreas; Mouse Spleen. ]# This antibody gave a positive result in IHC in the following FFPE tissue: Mouse spleen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab78294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 187 kDa (predicted molecular weight: 187 kDa). IHC-P Use a concentration of 5 µg/ml. Target
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Function
May be involved in the assembly, secretion and targeting of TGFB1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGFB1. May have a structural role in the extra cellular matrix (ECM). -
Tissue specificity
Isoform Long is found in fibroblasts. -
Sequence similarities
Belongs to the LTBP family.
Contains 18 EGF-like domains.
Contains 4 TB (TGF-beta binding) domains. -
Domain
Associates covalently with small latent TGF-beta complex via domain TB 3. -
Post-translational
modificationsContains hydroxylated asparagine residues.
Isoform Short N-terminus is blocked.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4052 Human
- Entrez Gene: 268977 Mouse
- Entrez Gene: 59107 Rat
- Omim: 150390 Human
- SwissProt: Q14766 Human
- SwissProt: Q8CG19 Mouse
- SwissProt: Q00918 Rat
- Unigene: 619315 Human
see all -
Alternative names
- 9430031G15Rik antibody
- 9830146M04 antibody
- Latent transforming growth factor beta binding protein 1 antibody
see all
Images
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Western blot - Anti-LTBP1 antibody (ab78294)All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 187 kDa
Observed band size: 187 kDa
Based on the immunogen sequence, this antibody is predicted to react with all three isoforms of LTBP1. The band detected here might correspond to the long isoform, which is 187 kDa and is found in fibroblasts. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP1 antibody (ab78294)IHC image of LTBP1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78294, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-LTBP1 antibody (ab78294)All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 187 kDa
Observed band size: 187 kDa
Additional bands at: 171 kDa (possible cleavage fragment), 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Protocols
References (6)
ab78294 has been referenced in 6 publications.
- Liu G et al. Fibulin-1c regulates transforming growth factor-ß activation in pulmonary tissue fibrosis. JCI Insight 5:N/A (2019). PubMed: 31343988
- Zhang H et al. iTRAQ-Based Quantitative Proteomic Comparison of Early- and Late-Passage Human Dermal Papilla Cell Secretome in Relation to Inducing Hair Follicle Regeneration. PLoS One 11:e0167474 (2016). IHC-P . PubMed: 27907131
- Horiguchi M et al. Abrogation of both short and long forms of latent transforming growth factor-ß binding protein-1 causes defective cardiovascular development and is perinatally lethal. Matrix Biol 43:61-70 (2015). PubMed: 25805620
- Hornigold N & Mooney A Extracellular matrix-induced Hic-5 expression in glomerular mesangial cells leads to a prosclerotic phenotype independent of TGF-ß. FASEB J 29:4956-67 (2015). PubMed: 26405299
- Maurya VK et al. Transforming growth factor-beta 1 (TGF-B1) liberation from its latent complex during embryo implantation and its regulation by estradiol in mouse. Biol Reprod 89:84 (2013). WB ; Mouse . PubMed: 23926286
- Squadrito ML et al. miR-511-3p modulates genetic programs of tumor-associated macrophages. Cell Rep 1:141-54 (2012). WB ; Mouse . PubMed: 22832163
Images
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Western blot - Anti-LTBP1 antibody (ab78294)All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 187 kDa
Observed band size: 187 kDa
Based on the immunogen sequence, this antibody is predicted to react with all three isoforms of LTBP1. The band detected here might correspond to the long isoform, which is 187 kDa and is found in fibroblasts. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP1 antibody (ab78294)
IHC image of LTBP1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78294, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-LTBP1 antibody (ab78294)All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 187 kDa
Observed band size: 187 kDa
Additional bands at: 171 kDa (possible cleavage fragment), 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
