Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LTBP1 antibody (ab78294)

Key features and details

  • Rabbit polyclonal to LTBP1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Overview

  • Product name

    Anti-LTBP1 antibody

  • Description

    Rabbit polyclonal to LTBP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human

  • Immunogen

    Synthetic peptide corresponding to Human LTBP1 aa 1350-1450 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab87128)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Rat Liver; Mouse Pancreas; Mouse Spleen. ]# This antibody gave a positive result in IHC in the following FFPE tissue: Mouse spleen.

Properties

Applications

Our Abpromise guarantee covers the use of ab78294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 187 kDa (predicted molecular weight: 187 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    May be involved in the assembly, secretion and targeting of TGFB1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGFB1. May have a structural role in the extra cellular matrix (ECM).

  • Tissue specificity

    Isoform Long is found in fibroblasts.

  • Sequence similarities

    Belongs to the LTBP family.
    Contains 18 EGF-like domains.
    Contains 4 TB (TGF-beta binding) domains.

  • Domain

    Associates covalently with small latent TGF-beta complex via domain TB 3.

  • Post-translational
    modifications

    Contains hydroxylated asparagine residues.
    Isoform Short N-terminus is blocked.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 9430031G15Rik antibody
    • 9830146M04 antibody
    • Latent transforming growth factor beta binding protein 1 antibody
    • Latent-transforming growth factor beta-binding protein 1 antibody
    • Latent-transforming growth factor beta-binding protein, isoform 1L antibody
    • Latent-transforming growth factor beta-binding protein, isoform 1S antibody
    • LBT1S antibody
    • LTBP-1 antibody
    • Ltbp1 antibody
    • LTBP1_HUMAN antibody
    • MGC163161 antibody
    • TGF-beta1-BP-1 antibody
    • Tgfb antibody
    • Transforming growth factor beta-1-binding protein 1 antibody
    • Transforming growth factor beta-1-masking protein large subunit antibody
    see all

Images

  • Western blot - Anti-LTBP1 antibody (ab78294)
    Western blot - Anti-LTBP1 antibody (ab78294)
    All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Pancreas (Mouse) Tissue Lysate
    Lane 3 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 187 kDa
    Observed band size: 187 kDa



    Based on the immunogen sequence, this antibody is predicted to react with all three isoforms of LTBP1. The band detected here might correspond to the long isoform, which is 187 kDa and is found in fibroblasts.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP1 antibody (ab78294)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP1 antibody (ab78294)

    IHC image of LTBP1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78294, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-LTBP1 antibody (ab78294)
    Western blot - Anti-LTBP1 antibody (ab78294)
    All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 187 kDa
    Observed band size: 187 kDa
    Additional bands at: 171 kDa (possible cleavage fragment), 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

References (6)

Publishing research using ab78294? Please let us know so that we can cite the reference in this datasheet.

ab78294 has been referenced in 6 publications.

  • Liu G  et al. Fibulin-1c regulates transforming growth factor-ß activation in pulmonary tissue fibrosis. JCI Insight 5:N/A (2019). PubMed: 31343988
  • Zhang H  et al. iTRAQ-Based Quantitative Proteomic Comparison of Early- and Late-Passage Human Dermal Papilla Cell Secretome in Relation to Inducing Hair Follicle Regeneration. PLoS One 11:e0167474 (2016). IHC-P . PubMed: 27907131
  • Horiguchi M  et al. Abrogation of both short and long forms of latent transforming growth factor-ß binding protein-1 causes defective cardiovascular development and is perinatally lethal. Matrix Biol 43:61-70 (2015). PubMed: 25805620
  • Hornigold N & Mooney A Extracellular matrix-induced Hic-5 expression in glomerular mesangial cells leads to a prosclerotic phenotype independent of TGF-ß. FASEB J 29:4956-67 (2015). PubMed: 26405299
  • Maurya VK  et al. Transforming growth factor-beta 1 (TGF-B1) liberation from its latent complex during embryo implantation and its regulation by estradiol in mouse. Biol Reprod 89:84 (2013). WB ; Mouse . PubMed: 23926286
  • Squadrito ML  et al. miR-511-3p modulates genetic programs of tumor-associated macrophages. Cell Rep 1:141-54 (2012). WB ; Mouse . PubMed: 22832163

Images

  • Western blot - Anti-LTBP1 antibody (ab78294)
    Western blot - Anti-LTBP1 antibody (ab78294)
    All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Pancreas (Mouse) Tissue Lysate
    Lane 3 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 187 kDa
    Observed band size: 187 kDa



    Based on the immunogen sequence, this antibody is predicted to react with all three isoforms of LTBP1. The band detected here might correspond to the long isoform, which is 187 kDa and is found in fibroblasts.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP1 antibody (ab78294)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP1 antibody (ab78294)

    IHC image of LTBP1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78294, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-LTBP1 antibody (ab78294)
    Western blot - Anti-LTBP1 antibody (ab78294)
    All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 187 kDa
    Observed band size: 187 kDa
    Additional bands at: 171 kDa (possible cleavage fragment), 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

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