Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)
![Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)](https://www.abcam.com/ps/products/247/ab247482/Images/ab247482-446603-anti-lrpap1-antibody-epr3327-bsa-and-azide-free-western-blot.jpg "Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3327] to LRPAP1 - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free
See all LRPAP1 primary antibodies -
Description
Rabbit monoclonal [EPR3327] to LRPAP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247482 is the carrier-free version of ab76500. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247482 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3327 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)All lanes : Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/6000 dilution (unpurified)
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This data was developed using ab76500, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)](https://www.abcam.com/ps/products/247/ab247482/Images/ab247482-446385-anti-lrpap1-antibody-epr3327-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)All lanes : Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/3000 dilution (purified)
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This data was developed using ab76500, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)](https://www.abcam.com/ps/products/247/ab247482/Images/ab247482-5-anti-lrpap1-antibody-epr3327-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)This data was developed using ab76500, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with unpurified ab76500 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)](https://www.abcam.com/ps/products/247/ab247482/Images/ab247482-4-anti-lrpap1-antibody-epr3327-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)This data was developed using ab76500, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with purified ab76500 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)](https://www.abcam.com/ps/products/247/ab247482/Images/ab247482-3-anti-lrpap1-antibody-epr3327-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)This data was developed using ab76500, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling LRPAP1 with unpurified ab76500 at a 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)](https://www.abcam.com/ps/products/247/ab247482/Images/ab247482-403520-anti-lrpap1-antibody-epr3327-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)This data was developed using ab76500, the same antibody clone in a different buffer formulation.
ab76500 (unpurified) at 1/300 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)](https://www.abcam.com/ps/products/247/ab247482/Images/ab247482-399568-anti-lrpap1-antibody-epr3327-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)This data was developed using ab76500, the same antibody clone in a different buffer formulation.
ab76500 (purified) at 1/150 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)](https://www.abcam.com/ps/products/247/ab247482/Images/ab247482-6-benefits-of-recombinant-antibodies.png)
Anti-LRPAP1 antibody [EPR3327] - BSA and Azide free (ab247482)
