Anti-LOK antibody - N-terminal (ab228802)
Key features and details
- Rabbit polyclonal to LOK - N-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LOK antibody - N-terminal
See all LOK primary antibodies -
Description
Rabbit polyclonal to LOK - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
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Immunogen
Recombinant fragment within Human LOK (N terminal). The exact sequence is proprietary.
Database link: O94804 -
Positive control
- WB: HeLa whole cell extract; A431 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LOK antibody - N-terminal (ab228802)All lanes : Anti-LOK antibody - N-terminal (ab228802) at 1/2000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : LOK Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 112 kDa
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Western blot - Anti-LOK antibody - N-terminal (ab228802)All lanes : Anti-LOK antibody - N-terminal (ab228802) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : LOK-knockout HeLa whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 112 kDa5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-LOK antibody - N-terminal (ab228802)Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for LOK (green) using ab228802 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOK antibody - N-terminal (ab228802)Paraffin-embedded human breast cancer tissue stained for LOK using ab228802 at 1/250 dilution in immunohistochemical analysis.
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Western blot - Anti-LOK antibody - N-terminal (ab228802)Anti-LOK antibody - N-terminal (ab228802) at 1/2000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 112 kDa5% SDS-PAGE gel.
