Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LMO2 antibody [SP51] - N-terminal (ab183328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP51] to LMO2 - N-terminal
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-LMO2 antibody [SP51] - N-terminal
    See all LMO2 primary antibodies

  • Description

    Rabbit monoclonal [SP51] to LMO2 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ramos cell lysate, Human tonsil tissue. Flow Cyt: Ramos and Raw264.7 cells

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    SP51

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling LMO2 with ab183328 at 1/50 dilution (3.22 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. The section was incubated with ab183328 for 30 mins at room temperature.

  • Western blot - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)
    Western blot - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)
    Anti-LMO2 antibody [SP51] - N-terminal (ab183328) at 1/25 dilution + Ramos cell lysate

    Predicted band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling LMO2 with ab183328 at 1/50 dilution (3.22 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183328 for 30 mins at room temperature.

  • Flow Cytometry - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)
    Flow Cytometry - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)

    Flow cytometry analysis of Raw264.7 (mouse abelson murine leukemia virus-induced tumor ) labeling LMO2 with purified ab183328 at 1/200 dilution (0.81 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Flow Cytometry - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)
    Flow Cytometry - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)

    Flow cytometry analysis of Ramos (human Burkitt's lymphoma) labeling LMO2 with purified ab183328 at 1/200 dilution (0.81 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling LMO2 with ab183328 at 1/100.

  • Flow Cytometry - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)
    Flow Cytometry - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)

    Flow cytometric analysis of LMO2 in RAMOS cells using ab183328 (1/100)(green) compared to negative control of rabbit IgG (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [SP51] - N-terminal (ab183328)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling LMO2 with ab183328 at 1/100.

  • Anti-LMO2 antibody [SP51] - N-terminal (ab183328)
    Anti-LMO2 antibody [SP51] - N-terminal (ab183328)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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