Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3725] to Lipin 1 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free
    See all Lipin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3725] to Lipin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247539 is the carrier-free version of ab92316 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247539 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    Western blot - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    All lanes : Anti-Lipin 1 antibody [EPR3725] (ab92316) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HuT-78 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 99 kDa



    This data was developed using ab92316, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    This data was developed using ab92316, the same antibody clone in a different buffer formulation.ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human colon tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    This data was developed using ab92316, the same antibody clone in a different buffer formulation.ab92316 at a 1/50 dilution, staining Lipin 1 in paraffin-embedded Human uterus tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    Immunocytochemistry - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    This data was developed using ab92316, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HepG2 cells using ab92316 at 1/100 dilution.
  • Flow Cytometry - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    Flow Cytometry - Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    This data was developed using ab92316, the same antibody clone in a different buffer formulation.Overlay histogram showing HepG2 cells stained with ab92316 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92316, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)
    Anti-Lipin 1 antibody [EPR3725] - BSA and Azide free (ab247539)

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