Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [FRAS92B] to LILRB3 - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free
    See all LILRB3 primary antibodies

  • Description

    Rat monoclonal [FRAS92B] to LILRB3 - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human non-hodgkin's lymphoma and tonsil tissue. ICC: 293T cells. Flow Cyt: 293T cells.

  • General notes

    ab271297 is the carrier-free version of ab271287. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab271297 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    FRAS92B

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)

    Immunohistochemical analysis of paraffin-embedded human non-hodgkin's lymphoma tissue labeling LILRB3 with ab271287 at 1.762µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human non-hodgkin's lymphoma. The section was incubated with ab271287 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271287).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LILRB3 with ab271287 at 1.762µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human tonsil. The section was incubated with ab271287 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271287).

  • Immunocytochemistry - Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)
    Immunocytochemistry - Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T cells labelling LILRB3 with ab271287 at 3.524µg/ml , followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
    Confocal image showing membranous and cytoplasmic staining on 293T cells transfected with LILRB3-myc plasmid.
    Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain Myc at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271287).

  • Flow Cytometry - Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)
    Flow Cytometry - Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with myc tagged LILRB3 construct cells labelling LILRB3 with ab271287 at 0.881µg/ml  (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271287).

  • Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)
    Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free (ab271297)

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