Anti-LIF antibody (ab113262)
Key features and details
- Rabbit polyclonal to LIF
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-LIF antibody
See all LIF primary antibodies -
Description
Rabbit polyclonal to LIF -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
A 16 amino acid synthetic peptide corresponding to internal sequence amino acids of Human LIF (NP_002300).
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Positive control
- WB: NIH/3T3, CaCo-2, Daudi, HeLa, Jurkat, SK-N-SH, and EL4 cell lysates. Mouse colon and rat lung tissue lysates; ICC/IF: NIH/3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab113262 is purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LIF antibody (ab113262)All lanes : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : EL4 (mouse lymphoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Diluting buffer: 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-LIF antibody (ab113262)Immunofluorescence analysis of 3T3 cells, staining LIF with ab113262 at 20 µg/ml. -
Western blot - Anti-LIF antibody (ab113262)Lane 1 : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 2 : Anti-LIF antibody (ab113262) at 2 µg/ml
All lanes : 3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa
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Western blot - Anti-LIF antibody (ab113262)Lane 1 : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 2 : Anti-LIF antibody (ab113262) at 2 µg/ml
All lanes : Rat lung tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Diluting buffer: 5% NFDM/TBST.
