Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] - BSA and Azide free (ab247861)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5712] to Leukotriene A4 hydrolase/LTA4H - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] - BSA and Azide free
    See all Leukotriene A4 hydrolase/LTA4H primary antibodies

  • Description

    Rabbit monoclonal [EPR5712] to Leukotriene A4 hydrolase/LTA4H - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, T-47D and A549 cell lysates.

  • General notes

    ab247861 is the carrier-free version of ab109434 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247861 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. This product was previously labelled as Leukotriene A4 hydrolase

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] - BSA and Azide free (ab247861)
    Western blot - Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] - BSA and Azide free (ab247861)
    All lanes : Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] (ab109434) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : LTA4H knockout HEK293T cell lysate
    Lane 3 : T-47D cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab109434).

    Lanes 1-4: Merged signal (red and green). Green - ab109434 observed at 69 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab109434 Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] was shown to specifically react with Leukotriene A4 hydrolase/LTA4H antibody in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266467 (knockout cell lysate ab258034) was used. Wild-type and Leukotriene A4 hydrolase/LTA4H antibody knockout samples were subjected to SDS-PAGE. ab109434 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] - BSA and Azide free (ab247861)
    Anti-Leukotriene A4 hydrolase/LTA4H antibody [EPR5712] - BSA and Azide free (ab247861)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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