Anti-LASP1 antibody (ab117806)
Key features and details
- Rabbit polyclonal to LASP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LASP1 antibody
See all LASP1 primary antibodies -
Description
Rabbit polyclonal to LASP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 100-150 of Human LASP1 (NP_006139.1).
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Positive control
- Whole cell lysate from HeLa, 293T and Jurkat cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab117806 was affinity purified using an epitope specific to LASP1 immobilized on a solid support . -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LASP1 antibody (ab117806)All lanes : Anti-LASP1 antibody (ab117806) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-LASP1 antibody (ab117806)ab117806 at 1 µg/ml staining LASP1 by WB following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab117806 at 6 µg/mg lysate.
Lane 2; IP using an antibody to a downstream epitope.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.
