Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 117 264 ₸

Order now and get it on Wednesday March 31, 2021

Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23810-121] to L3MBTL1
Suitable for: ICC, WB, IP, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Product name

Anti-L3MBTL1 antibody [EPR23810-121]
See all L3MBTL1 primary antibodies
Description

Rabbit monoclonal [EPR23810-121] to L3MBTL1
Host species

Rabbit
Tested applications

Suitable for: ICC, WB, IP, Flow Cyt (Intra)more details
Unsuitable for: IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: IMR-32, SH-SY5Y, SW480, Neuro-2a, NIH/3T3, C6, 293T, HeLa lysates. ICC: IMR-32 cells. Flow Cyt: IMR-32 cell. IP: IMR-32, Neuro-2a cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23810-121
Isotype

IgG

Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

All lanes : Anti-L3MBTL1 antibody [EPR23810-121] (ab259919) at 1/1000 dilution

Lane 1 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HL-60 (human Acute Promyelocytic Leukemia promyeloblast) whole cell lysate
Lane 5 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 7 : C6 (rat glial tumor glial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 83 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: HL-60 (PMID: 10445843).

The two reactive bands represent different isoforms

Exposure time: 37 seconds
Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

All lanes : Anti-L3MBTL1 antibody [EPR23810-121] (ab259919) at 1/1000 dilution

Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 83 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 10 seconds
Immunocytochemistry - (ab259919)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized IMR-32 cells labelling L3MBTL1 with ab259919 at 1/50 (10.4 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in IMR-32 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Flow Cytometry - (ab259919)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized IMR-32 (human neuroblastoma neuroblast) cells labelling L3MBTL1 with ab259919 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - (ab259919)

L3MBTL1 was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 ug with ab259919 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259919 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 ug

Lane 2: ab259919 IP in IMR-32 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259919 in IMR-32 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 5.5 seconds
Immunoprecipitation - (ab259919)

L3MBTL1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug with ab259919 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259919 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug

Lane 2: ab259919 IP in Neuro-2a whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259919 in Neuro-2a whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 5.5 seconds
(ab259919)

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