Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23810-121] to L3MBTL1
  • Suitable for: ICC, WB, IP, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-L3MBTL1 antibody [EPR23810-121]
    See all L3MBTL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23810-121] to L3MBTL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IP, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: IMR-32, SH-SY5Y, SW480, Neuro-2a, NIH/3T3, C6, 293T, HeLa lysates. ICC: IMR-32 cells. Flow Cyt: IMR-32 cell. IP: IMR-32, Neuro-2a cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23810-121

  • Isotype

    IgG

Images

  • Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)
    Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)
    All lanes : Anti-L3MBTL1 antibody [EPR23810-121] (ab259919) at 1/1000 dilution

    Lane 1 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate
    Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
    Lane 3 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : HL-60 (human Acute Promyelocytic Leukemia promyeloblast) whole cell lysate
    Lane 5 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 6 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 7 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 83 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: HL-60 (PMID: 10445843).

    The two reactive bands represent different isoforms

     Exposure time: 37 seconds

     

  • Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)
    Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)
    All lanes : Anti-L3MBTL1 antibody [EPR23810-121] (ab259919) at 1/1000 dilution

    Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 83 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     Exposure time: 10 seconds

     

  • Immunocytochemistry - (ab259919)
    Immunocytochemistry - (ab259919)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized IMR-32 cells labelling L3MBTL1 with ab259919 at 1/50 (10.4 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in IMR-32 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - (ab259919)
    Flow Cytometry - (ab259919)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized IMR-32 (human neuroblastoma neuroblast) cells labelling L3MBTL1 with ab259919 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - (ab259919)
    Immunoprecipitation - (ab259919)

    L3MBTL1 was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 ug with ab259919 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259919 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 ug

    Lane 2: ab259919 IP in IMR-32 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259919 in IMR-32 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds

     

  • Immunoprecipitation - (ab259919)
    Immunoprecipitation - (ab259919)

    L3MBTL1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug with ab259919 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259919 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug

    Lane 2: ab259919 IP in Neuro-2a whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259919 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds

     

  • (ab259919)
    (ab259919)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.