Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Kv4.3/KCND3 antibody [K75/41] (ab252538)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [K75/41] to Kv4.3/KCND3
Suitable for: IHC-P
Reacts with: Mouse, Rat

Product name

Anti-Kv4.3/KCND3 antibody [K75/41]
See all Kv4.3/KCND3 primary antibodies
Description

Mouse monoclonal [K75/41] to Kv4.3/KCND3
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Mouse cerebrum tissue; Rat cerebrum tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

K75/41
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (ab252538)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the mouse cerebrum. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (ab252538)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the rat cerebrum. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (ab252538)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on the mouse skeletal muscle. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (ab252538)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on the rat skeletal muscle. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Anti-Kv4.3/KCND3 antibody [K75/41] (ab252538)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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