Anti-Ku80 antibody [5C5] (ab119935)
![Anti-Ku80 antibody [5C5] (ab119935)](https://www.abcam.com/ps/products/119/ab119935/Images/ab119935-133486-anti-ku80-antibody-5c5-western-blot.jpg "Anti-Ku80 antibody [5C5] (ab119935)")
Key features and details
- Mouse monoclonal [5C5] to Ku80
- Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Ku80 antibody [5C5]
See all Ku80 primary antibodies -
Description
Mouse monoclonal [5C5] to Ku80 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment, corresponding to amino acids 61-340 of Human Ku80
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Positive control
- HeLa, MCF7, A549 and NIH3T3 cell lysates, Human tonsil and Human colon cancer tissues, Hela cells, recombinant Ku80 protein
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR133722-19 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5C5 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Ku80 antibody [5C5] (ab119935)All lanes : Anti-Ku80 antibody [5C5] (ab119935) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : NIH3T3 cell lysate
Predicted band size: 83 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody [5C5] (ab119935)](https://www.abcam.com/ps/products/119/ab119935/Images/ab119935-133485-anti-ku80-antibody-5c5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody [5C5] (ab119935)Immunohistochemical analysis of paraffin-embedded Human tonsil tissues using ab119935 at a dilution of 1/200 with DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody [5C5] (ab119935)](https://www.abcam.com/ps/products/119/ab119935/Images/ab119935-133484-anti-ku80-antibody-5c5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku80 antibody [5C5] (ab119935)Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using ab119935 at a dilution of 1/200 with DAB staining. -
![Immunocytochemistry/ Immunofluorescence - Anti-Ku80 antibody [5C5] (ab119935)](https://www.abcam.com/ps/products/119/ab119935/Images/ab119935-133483-anti-ku80-antibody-5c5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ku80 antibody [5C5] (ab119935)Immunofluorescence analysis of Hela cells using ab119935 at a dilution of 1/200 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. -
![Flow Cytometry - Anti-Ku80 antibody [5C5] (ab119935)](https://www.abcam.com/ps/products/119/ab119935/Images/ab119935-133482-anti-ku80-antibody-5c5-flow-cytometry.jpg)
Flow Cytometry - Anti-Ku80 antibody [5C5] (ab119935)Flow cytometric analysis of Hela cells using ab119935 at a dilution of 1/200 (green) and negative control (purple). -
![ELISA - Anti-Ku80 antibody [5C5] (ab119935)](https://www.abcam.com/ps/products/119/ab119935/Images/ab119935-133481-anti-ku80-antibody-5c5-elisa.jpg)
ELISA - Anti-Ku80 antibody [5C5] (ab119935)ELISA, using ab119935 at a dilution of 1/10000.
