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Anti-KPNB1 antibody [OTI6H3] (ab236508)

Anti-KPNB1 antibody [OTI6H3] (ab236508)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI6H3] to KPNB1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-KPNB1 antibody [OTI6H3]
    See all KPNB1 primary antibodies
  • Description

    Mouse monoclonal [OTI6H3] to KPNB1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human KPNB1 aa 1-200. (NP_002256) produced in E.coli.
    Database link: Q14974

  • Positive control

    • WB: HEK-293T transfected with pCMV6-ENTRY KPNB1; HeLa, A431, NIH/3T3 and PC-12 whole cell lysates. IHC-P: Human adult heart tissue.
  • General notes

    Previously labelled as NTF97/Importin beta. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    OTI6H3
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Envelope
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Images

  • Western blot - Anti-KPNB1 antibody [OTI6H3] (ab236508)
    Western blot - Anti-KPNB1 antibody [OTI6H3] (ab236508)
    All lanes : Anti-KPNB1 antibody [OTI6H3] (ab236508) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY KPNB1

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 97 kDa

  • Western blot - Anti-KPNB1 antibody [OTI6H3] (ab236508)
    Western blot - Anti-KPNB1 antibody [OTI6H3] (ab236508)
    All lanes : Anti-KPNB1 antibody [OTI6H3] (ab236508) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 97 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [OTI6H3] (ab236508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNB1 antibody [OTI6H3] (ab236508)

    Paraffin-embedded human adult heart tissue stained for KPNB1 using ab236508 at 1/2000 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: IHC-P, IP, WB

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