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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation

Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350)

Price and availability

307 566 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20927-67] to KMT3A / HYPB / HIF-1
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67]
    See all KMT3A / HYPB / HIF-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20927-67] to KMT3A / HYPB / HIF-1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MEF and HepG2 whole cell lysates. HeLa nuclear fraction.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20927-67
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine methylation

Images

  • Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350)
    Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350)
    All lanes : Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350) at 1/1000 dilution

    Lane 1 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 288 kDa
    Observed band size: 288 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane 1: 26 seconds; Lane 2: 86 seconds.  

  • Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350)
    Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350)
    Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350) at 1/1000 dilution + HeLa (human cervix adenocarcinoma epithelial cell) nuclear fraction at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 288 kDa
    Observed band size: 288 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350)
    Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (ab239350)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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