Anti-KMT1B / SUV39H2 antibody - C-terminal (ab71683)
Key features and details
- Rabbit polyclonal to KMT1B / SUV39H2 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KMT1B / SUV39H2 antibody - C-terminal
See all KMT1B / SUV39H2 primary antibodies -
Description
Rabbit polyclonal to KMT1B / SUV39H2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human KMT1B/ SUV39H2 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q9H5I1 -
Positive control
- 293 and HL60 cell line lysates. Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab71683 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KMT1B / SUV39H2 antibody - C-terminal (ab71683)All lanes : Anti-KMT1B / SUV39H2 antibody - C-terminal (ab71683)
Lane 1 : 293 cell line lysates
Lane 2 : HL60 cell line lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody - C-terminal (ab71683)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ab71683 at 1/50 dilution. A peroxidase-conjugated secondary antibody was used, followed by DAB staining. -
Immunocytochemistry/ Immunofluorescence - Anti-KMT1B / SUV39H2 antibody - C-terminal (ab71683)ICC/IF image of ab71683 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71683, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
