Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13484] to KLHL6 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-KLHL6 antibody [EPR13484] - BSA and Azide free
    See all KLHL6 primary antibodies

  • Description

    Rabbit monoclonal [EPR13484] to KLHL6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250605 is the carrier-free version of ab182163. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250605 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)
    Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)
    This data was developed using ab182163, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab182163 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
  • Immunocytochemistry - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)
    Immunocytochemistry - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)
    This data was developed using ab182163, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling KLHL6 with ab182163 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI.
  • Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)
    Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)
    Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + A549 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    This data was developed using ab182163, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)
    Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)
    Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    This data was developed using ab182163, the same antibody clone in a different buffer formulation.

     

  • Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)
    Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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