Anti-KLB antibody (ab106794)
Key features and details
- Rabbit polyclonal to KLB
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KLB antibody
See all KLB primary antibodies -
Description
Rabbit polyclonal to KLB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human KLB aa 700-800 conjugated to keyhole limpet haemocyanin.
(Peptide available asab127879) -
Positive control
- This antibody gave a positive signal in HepG2 whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal stomach.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanAll applications Macaque monkeyGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 120 kDa).IHC-P Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 120 kDa).IHC-P
Use a concentration of 5 µg/ml.Target
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Function
Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. -
Sequence similarities
Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. -
Domain
Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 241, and the second one lacks the essential Glu active site residue at position 889. These domains are therefore predicted to be inactive. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 152831 Human
- Omim: 611135 Human
- SwissProt: Q86Z14 Human
- Unigene: 90756 Human
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Alternative names
- Beta-klotho antibody
- BetaKlotho antibody
- BKL antibody
see all
Images
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Western blot - Anti-KLB antibody (ab106794)Anti-KLB antibody (ab106794) at 1 µg/ml + HepG2 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
KLB contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLB antibody (ab106794)IHC image of KLB staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106794, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References (6)
ab106794 has been referenced in 6 publications.
- Ye L et al. FGF21 promotes functional recovery after hypoxic-ischemic brain injury in neonatal rats by activating the PI3K/Akt signaling pathway via FGFR1/ß-klotho. Exp Neurol 317:34-50 (2019). PubMed: 30802446
- Jackson TC et al. Robust RBM3 and ß-klotho expression in developing neurons in the human brain. J Cereb Blood Flow Metab 39:2355-2367 (2019). PubMed: 31566073
- Wang D et al. Decreased placental and muscular expression of the fibroblast growth factor 19 in gestational diabetes mellitus. J Diabetes Investig N/A:N/A (2018). PubMed: 29734515
- Liu Z et al. ßKlotho inhibits androgen/androgen receptor-associated epithelial-mesenchymal transition in prostate cancer through inactivation of ERK1/2 signaling. Oncol Rep 40:217-225 (2018). PubMed: 29749458
- Jackson TC et al. Infants Uniquely Express High Levels of RBM3 and Other Cold-Adaptive Neuroprotectant Proteins in the Human Brain. Dev Neurosci 40:325-336 (2018). PubMed: 30399610
- Dekker Nitert M et al. Increased placental expression of fibroblast growth factor 21 in gestational diabetes mellitus. J Clin Endocrinol Metab 99:E591-8 (2014). PubMed: 24432989
Images
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Western blot - Anti-KLB antibody (ab106794)Anti-KLB antibody (ab106794) at 1 µg/ml + HepG2 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
KLB contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLB antibody (ab106794)
IHC image of KLB staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106794, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
