Anti-KDM3A / JHDM2A antibody (ab80598)
Key features and details
- Rabbit polyclonal to KDM3A / JHDM2A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KDM3A / JHDM2A antibody
See all KDM3A / JHDM2A primary antibodies -
Description
Rabbit polyclonal to KDM3A / JHDM2A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 1271-1321 of human KDM3A/ JHDM2A
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified using an epitope specific to KDM3A / JHDM2A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KDM3A / JHDM2A antibody (ab80598)All lanes : Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/ml
Lane 1 : whole HeLa cell lysate at 50 µg
Lane 2 : whole HeLa cell lysate at 15 µg
Lane 3 : whole HeLa cell lysate at 5 µg
Predicted band size: 147 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (ab80598)Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Lane 1: ab80598 used for IP at 3 µg/mg lysate; Lane 2: a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 µg/ml.
