Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16312] to KCTD5 - C-terminal
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KCTD5 antibody [EPR16312] - C-terminal
    See all KCTD5 primary antibodies

  • Description

    Rabbit monoclonal [EPR16312] to KCTD5 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Positive control

    • HeLa, Jurkat, RAW 264.7, C6 and NIH 3T3 whole cell lysates; Human kidney and Rat liver tissues; A431 cells; Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16312

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
    Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
    All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysates
    Lane 2 : Jurkat whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml).  A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194825.
    Note: Both cytoplasm and nuclear staining on human kidney tissue.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
    Western blot - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
    All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution

    Lane 1 : C6 whole cell lysates
    Lane 2 : RAW 264.7 whole cell lysates
    Lane 3 : NIH 3T3 whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194825.
    Note: Both cytoplasm and nuclear staining on rat liver tissue.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
    Immunocytochemistry/ Immunofluorescence - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml), followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Counterstained with Dapi (blue). Both cytoplasm and nuclear staining on A431 cell line is observed.

  • Flow Cytometry - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
    Flow Cytometry - Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

    Flow cytometric analysis of Jurkat cells labeling KCTD5 with ab194825 at 1/70 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).

  • Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)
    Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.