Anti-KBTB6/KBTBD6 antibody (ab222971)
Key features and details
- Rabbit polyclonal to KBTB6/KBTBD6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KBTB6/KBTBD6 antibody -
Description
Rabbit polyclonal to KBTB6/KBTBD6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human KBTB6/KBTBD6 aa 1-150.
Database link: Q86V97 -
Positive control
- WB: HeLa cell lysate; mouse liver, kidney and brain tissue lysates. IHC-P: Human liver tissue. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as KBTB6
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KBTB6/KBTBD6 (green) using ab222971 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KBTB6/KBTBD6 antibody (ab222971)Paraffin-embedded human liver tissue stained for KBTB6/KBTBD6 using ab222971 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-KBTB6/KBTBD6 antibody (ab222971) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse brain tissue lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 76 kDa
Observed band size: 50,77 kDa why is the actual band size different from the predicted?

