Anti-KAT13C / NCOA2 antibody (ab2842)
Key features and details
- Rabbit polyclonal to KAT13C / NCOA2
- Suitable for: ICC/IF
- Reacts with: Human, Chinese hamster
- Isotype: IgG
Overview
-
Product name
Anti-KAT13C / NCOA2 antibody
See all KAT13C / NCOA2 primary antibodies -
Description
Rabbit polyclonal to KAT13C / NCOA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanChinese hamster
-
Immunogen
Synthetic peptide corresponding to Human KAT13C/ NCOA2 aa 206-217.
Sequence:PLPDSEEEGHDS
(Peptide available asab4917) -
General notes
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Glucocorticoid receptor interacting protein-1 (GRIP 1), or Transcription Intermediary Factor 2 (TIF 2), is a member of a family of transcriptional coactivator proteins which includes steroid receptor coactivator-1 (SRC 1) and Amplified in breast cancer-1 (AIB 1). GRIP1 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, estrogen, progesterone, and peroxisome proliferator activated receptors. GRIP 1 also contains several copies of the conserved LXXLL/LLXXL motif which has been demonstrated to be critical to receptor-coactivator interactions. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-KAT13C / NCOA2 antibody (ab2842)
Immunofluorescent analysis of PC-3 (Human prostate adenocarcinoma cell line) cells on 70% confluent log phase labeling KAT13C / NCOA2. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab2842 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
-
Immunocytochemistry/ Immunofluorescence - Anti-KAT13C / NCOA2 antibody (ab2842)Immunolocalization of KAT13C/NCOA2 in CHO (Chinese hamster ovary cell line) cells using ab2842.
