Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23570-293] to Kallikrein 14 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free
    See all Kallikrein 14 primary antibodies

  • Description

    Rabbit monoclonal [EPR23570-293] to Kallikrein 14 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse esophagus tissue lysate; Mouse Kallikrein 14 recombinant protein (aa24-250). IHC-P: Mouse esophagus. IHC-Fr: Mouse esophagus.

  • General notes

    ab278506 is the carrier-free version of ab278500. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278506 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)
    Western blot - Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)
    All lanes : Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500) at 1/1000 dilution

    Lane 1 : Mouse esophagus tissue lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 29 kDa



    This data was developed using ab278500, the same antibody clone in a different buffer formulation.

    Blocking buffer and Diluting buffer : 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID: 17110383).

    Negative control: kidney (PMID: 11352573)

    Exposure time: 26 seconds.

  • Western blot - Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)
    Western blot - Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)
    Anti-Kallikrein 14 antibody [EPR23570-293] (ab278500) at 1/1000 dilution + Mouse Kallikrein 14 recombinant protein (aa24-250) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 29 kDa



    This data was developed using ab278500, the same antibody clone in a different buffer formulation.

    Blocking buffer and Diluting buffer : 5% NFDM/TBST

    Exposure: 10 seconds 

    Mouse Kallikrein 14 recombinant protein (aa24-250) was expressed from a mammalian - HEK-293 expression system.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)

    This data was developed using ab278500, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse esophagus tissue labelling Thyroid Peroxidase/TPO with ab278500 at 1/5000 (0.585 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. The section was incubated with ab278500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)
    Immunohistochemistry (Frozen sections) - Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)

    This data was developed using ab278500, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse esophagus tissue labelling kallikrein 14 with ab278500 at 1/100 (5.85 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 14 antibody [EPR23570-293] - BSA and Azide free (ab278506)

    This data was developed using ab278500, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling Thyroid Peroxidase/TPO with ab278500 at 1/5000 (0.585 ug/ml) dilution  followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. The section was incubated with ab278500 for 30 mins at room temperature. Negative control: No staining on mouse kidney. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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