Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17365] to JunD - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-JunD antibody [EPR17365] - BSA and Azide free
    See all JunD primary antibodies

  • Description

    Rabbit monoclonal [EPR17365] to JunD - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250511 is the carrier-free version of ab181615.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

    Lane 1 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
    Lane 3 : Human fetal liver lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39,42 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab181615, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39,42 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab181615, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39,42 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using ab181615, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Western blot - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate
    Lane 3 : PC12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
    Lane 4 : NIH 3T3 (Mouse embyro fibroblast cells ) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39,42 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab181615, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    This data was developed using ab181615, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
    Note: Nuclear staining on the epithelial cells of Human mammary gland was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    This data was developed using ab181615, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung squamous cell carcinoma tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
    Note: Nucleus staining on the cancer cells of lung squamous cell carcinoma was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    This data was developed using ab181615, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
    Note: Nuclear staining on neurons of the mouse cerebral cortex was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    This data was developed using ab181615, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
    Note: Nuclear staining on neurons of the rat cerebral cortex was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Immunocytochemistry - Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    This data was developed using ab181615, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, labeling JunD with ab181615 at 1/10000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image shows nuclear staining on the HeLa cell line. The nuclear counter stain is DAPI (blue) . Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab181615 at 1/10000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
    Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)

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