Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab240742)
Key features and details
- Rabbit polyclonal to Junctional Adhesion Molecule 1/JAM-A
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Junctional Adhesion Molecule 1/JAM-A antibody
See all Junctional Adhesion Molecule 1/JAM-A primary antibodies -
Description
Rabbit polyclonal to Junctional Adhesion Molecule 1/JAM-A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Junctional Adhesion Molecule 1/JAM-A aa 249-299. The exact sequence is proprietary. NP_058642.1 (GeneID 50848).
Database link: Q9Y624 -
Positive control
- WB: HEK-293T and HeLa whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Human prostate carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Junctional Adhesion Molecule 1/JAM-A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab240742)All lanes : Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab240742) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab240742)Junctional Adhesion Molecule 1/JAM-A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab240742 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab240742 at 1 μg/mL.
Lane 1: ab240742 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure times of 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab240742)Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Junctional Adhesion Molecule 1/JAM-A using ab240742 at 1/200 dilution in immunohistochemical analysis. DAB staining.
