Anti-JHD1 antibody (ab39335)
Key features and details
- Rabbit polyclonal to JHD1
- Suitable for: WB
- Isotype: IgG
Overview
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Product name
Anti-JHD1 antibody -
Description
Rabbit polyclonal to JHD1This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Species reactivity
Predicted to work with: Saccharomyces cerevisiae
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Immunogen
Synthetic peptide corresponding to Saccharomyces cerevisiae JHD1 aa 200-300.
(Peptide available asab39334)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
Application Abreviews Notes WB Use at an assay dependent concentration. Predicted molecular weight: 133 kDa.Notes WB
Use at an assay dependent concentration. Predicted molecular weight: 133 kDa.Target
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Relevance
KDM2 / JHD1 is a JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 856777 Saccharomyces cerevisiae
- SwissProt: P40034 Saccharomyces cerevisiae
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Alternative names
- JHD1 antibody
- Jhd1p antibody
- JmjC domain containing histone demethylation protein 1 antibody
- Jumonji/ARID domain containing protein 1 antibody
Images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.-
Western blot - Anti- JHD1 antibody (ab39335)All lanes : Anti-JHD1 antibody (ab39335) at 1 µg/ml
Lane 1 : s.cerevisiae
Lane 2 : s.cerevisiae with S. cerevisiae JHD1 peptide (ab39334) at 1 µg/ml
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 34 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab39335 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti- JHD1 antibody (ab39335)All lanes : Anti-JHD1 antibody (ab39335) at 1 µg/ml
Lane 1 : s.cerevisiae
Lane 2 : s.cerevisiae with S. cerevisiae JHD1 peptide (ab39334) at 1 µg/ml
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 34 kDa, 90 kDa. We are unsure as to the identity of these extra bands.