Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ITPA antibody [EPR8780] - BSA and Azide free (ab248742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8780] to ITPA - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ITPA antibody [EPR8780] - BSA and Azide free
    See all ITPA primary antibodies

  • Description

    Rabbit monoclonal [EPR8780] to ITPA - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T and Jurkat cell lysates.

  • General notes

    ab248742 is the carrier-free version of ab134937 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248742 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Inosine triphosphate pyrophosphatase

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ITPA antibody [EPR8780] - BSA and Azide free (ab248742)
    Western blot - Anti-ITPA antibody [EPR8780] - BSA and Azide free (ab248742)
    All lanes : Anti-ITPA antibody [EPR8780] (ab134937) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 21 kDa



    This data was developed using ab134937, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPA antibody [EPR8780] - BSA and Azide free (ab248742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPA antibody [EPR8780] - BSA and Azide free (ab248742)
    This data was developed using ab134937, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human breast carcinoma tissue, labelling ITPA using ab134937 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-ITPA antibody [EPR8780] - BSA and Azide free (ab248742)
    Western blot - Anti-ITPA antibody [EPR8780] - BSA and Azide free (ab248742)
    All lanes : Anti-ITPA antibody [EPR8780] (ab134937) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ITPA knockout HEK293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab134937).

    Lanes 1-3: Merged signal (red and green). Green - ab134937 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab134937 Anti-ITPA antibody [EPR8780]  was shown to specifically react with ITPA in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266679 (knockout cell lysate ab258477) was used. Wild-type and ITPA knockout samples were subjected to SDS-PAGE. ab134937 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-ITPA antibody [EPR8780] - BSA and Azide free (ab248742)
    Anti-ITPA antibody [EPR8780] - BSA and Azide free (ab248742)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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