Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y401] to ITK/EMT - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human

Overview

  • Product name

    Anti-ITK/EMT antibody [Y401] - BSA and Azide free
    See all ITK/EMT primary antibodies

  • Description

    Rabbit monoclonal [Y401] to ITK/EMT - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat whole cell lysate and MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate. Flow Cyt (Intra): Jurkat cells.

  • General notes

    ab157243 is the carrier-free version of ab32039.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Western blot - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
    Western blot - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
    Anti-ITK/EMT antibody [Y401] (ab32039) at 1/5000 dilution + Jurkat cell lysate

    Predicted band size: 72 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32039, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
    This data was developed using ab32039, the same antibody clone in a different buffer formulation.ab32039 at a 1/250 dilution, staining ITK/EMT in paraffin embedded human lymph node tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
    Flow Cytometry - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
    This data was developed using ab32039, the same antibody clone in a different buffer formulation.Overlay histogram showing Jurkat cells stained with ab32039 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32039, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
    Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)

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