Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
![Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)](https://www.abcam.com/ps/products/157/ab157243/Images/ab157243-405349-anti-itk-emt-antibody-y401-bsa-and-azide-free-western-blot.gif "Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y401] to ITK/EMT - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Overview
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Product name
Anti-ITK/EMT antibody [Y401] - BSA and Azide free
See all ITK/EMT primary antibodies -
Description
Rabbit monoclonal [Y401] to ITK/EMT - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat whole cell lysate and MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate. Flow Cyt (Intra): Jurkat cells.
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General notes
ab157243 is the carrier-free version of ab32039.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y401 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)Anti-ITK/EMT antibody [Y401] (ab32039) at 1/5000 dilution + Jurkat cell lysate
Predicted band size: 72 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?This data was developed using ab32039, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)](https://www.abcam.com/ps/products/157/ab157243/Images/ab157243-2-anti-itk-emt-antibody-y401-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)This data was developed using ab32039, the same antibody clone in a different buffer formulation.ab32039 at a 1/250 dilution, staining ITK/EMT in paraffin embedded human lymph node tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Flow Cytometry - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)](https://www.abcam.com/ps/products/157/ab157243/Images/ab157243-1-anti-itk-emt-antibody-y401-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)This data was developed using ab32039, the same antibody clone in a different buffer formulation.Overlay histogram showing Jurkat cells stained with ab32039 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32039, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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![Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)](https://www.abcam.com/ps/products/157/ab157243/Images/ab157243-4-benefits-of-recombinant-antibodies.png)
Anti-ITK/EMT antibody [Y401] - BSA and Azide free (ab157243)
