Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tec antibody [Y398] - BSA and Azide free (ab229196)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y398] to Tec - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Tec antibody [Y398] - BSA and Azide free
    See all Tec primary antibodies

  • Description

    Rabbit monoclonal [Y398] to Tec - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab229196 reacts with an epitope located in the PH domain of Tec.

  • Positive control

    • IHC-P: Human ovary carcinoma tissue. ICC/IF: Jurkat cells. Flow Cyt (intra): Jurkat cells.

  • General notes

    ab229196 is the carrier-free version of ab32368.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Tec antibody [Y398] - BSA and Azide free (ab229196)
    Immunocytochemistry/ Immunofluorescence - Anti-Tec antibody [Y398] - BSA and Azide free (ab229196)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) labeling Tec with purified ab32368 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32368).

  • Flow Cytometry - Anti-Tec antibody [Y398] - BSA and Azide free (ab229196)
    Flow Cytometry - Anti-Tec antibody [Y398] - BSA and Azide free (ab229196)

    Overlay histogram showing Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained with ab32368 (red line).

    The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32368, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32368).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tec antibody [Y398] - BSA and Azide free (ab229196)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tec antibody [Y398] - BSA and Azide free (ab229196)

    ab32368, at a 1/50 dilution staining human ovary carcinoma tissue by immunohistochemistry, paraffin embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32368).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Tec antibody [Y398] - BSA and Azide free (ab229196)
    Anti-Tec antibody [Y398] - BSA and Azide free (ab229196)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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