Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ITCH/AIP4 antibody [EPR4936] (ab108515)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4936] to ITCH/AIP4
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ITCH/AIP4 antibody [EPR4936]
    See all ITCH/AIP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR4936] to ITCH/AIP4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, HeLa, HAP1 and 293T cell lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4936

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ITCH/AIP4 antibody [EPR4936] (ab108515)
    Western blot - Anti-ITCH/AIP4 antibody [EPR4936] (ab108515)
    All lanes : Anti-ITCH/AIP4 antibody [EPR4936] (ab108515) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ITCH knockout HeLa cell lysate
    Lane 3 : HAP1 cell lysate
    Lane 4 : K-562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 103 kDa
    Observed band size: 103 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab108515 observed at 103 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab108515 Anti-ITCH/AIP4 antibody [EPR4936] was shown to specifically react with ITCH/AIP4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265338 (knockout cell lysate ab258014) was used.  Wild-type and ITCH/AIP4 knockout samples were subjected to SDS-PAGE.  ab108515 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ITCH/AIP4 antibody [EPR4936] (ab108515)
    Western blot - Anti-ITCH/AIP4 antibody [EPR4936] (ab108515)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: ITCH/AIP4 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: K562 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab108515 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab108515 was shown to specifically react with ITCH/AIP4 when ITCH/AIP4 knockout samples were used. Wild-type and ITCH/AIP4 knockout samples were subjected to SDS-PAGE. ab108515 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ITCH/AIP4 antibody [EPR4936] (ab108515)
    Western blot - Anti-ITCH/AIP4 antibody [EPR4936] (ab108515)
    All lanes : Anti-ITCH/AIP4 antibody [EPR4936] (ab108515) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 103 kDa

  • Anti-ITCH/AIP4 antibody [EPR4936] (ab108515)
    Anti-ITCH/AIP4 antibody [EPR4936] (ab108515)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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