Human ITCH (AIP4) knockout HeLa cell lysate (ab258014)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab262281 - Human ITCH knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human ITCH knockout HeLa cell lysate (ab258014)
Lane 1:Wild-type HeLa cell lysate (20 ug)
Lane 2:ITCH knockout HeLa cell lysate (20 ug)
Lane 3:HAP1 cell lysate (20 ug)
Lane 4:K-562 cell lysate (20 ug)ab108515 was shown to specifically react with ITCH/AIP4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265338 (knockout cell lysate ab258014) was used. Wild-type and ITCH/AIP4 knockout samples were subjected to SDS-PAGE. ab108515 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human ITCH knockout HeLa cell lysate (ab258014)Homozygous: 1 bp insertion in exon7
