Anti-IST1 antibody (ab244502)
Key features and details
- Rabbit polyclonal to IST1
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IST1 antibody -
Description
Rabbit polyclonal to IST1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
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Immunogen
Recombinant fragment within Human IST1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P53990 -
Positive control
- IHC-P: Human testis, endometrium, and duodenum tissues; WB: NTERA-2 cell lysate. ICC: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IST1 antibody (ab244502)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling IST1 with ab244502 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-IST1 antibody (ab244502)PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for IST1 (green) using ab244502 at 4 µg/ml in ICC.
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Western blot - Anti-IST1 antibody (ab244502)All lanes : Anti-IST1 antibody (ab244502) at 0.4 µg/ml
Lane 1 : NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 2 : SK-BR-3 (human mammary gland adenocarcinoma cell line) whole cell lysate
Predicted band size: 40 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IST1 antibody (ab244502)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling IST1 with ab244502 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IST1 antibody (ab244502)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human duodenum tissue labelling IST1 with ab244502 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IST1 antibody (ab244502)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IST1 with ab244502 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.