Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
Suitable for: ICC/IF, WB, IP, Flow Cyt
Reacts with: Mouse, Chicken, Human

Product name

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
See all Islet 1 primary antibodies
Description

Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
Host species

Rabbit
Specificity

86% identities with Islet 2

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Mouse Chicken Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562, HeLa, Jurkat and SH-SY5Y cell lysates. IHC-Fr: Chicken hindbrain tissue. ICC/IF: U-87 MG cell line.
General notes
Insulin gene enhancer protein ISL-1 is a protein encoded by the ISL1 gene. Among other roles, ISL-1 plays a part in the embryogenesis of pancreatic islets of Langerhans and some models, deficiency have shown failure of neural tube motor neurons to differentiate.

Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP4182
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

All lanes : Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (unpurified)

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 39 kDa
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 39 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (purified) + UMNSAH/DF-1 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 39 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.
Flow Cytometry - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution(10 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 39 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 39 kDa

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 µg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

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