Anti-IRX5 antibody (ab100939)
Key features and details
- Rabbit polyclonal to IRX5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IRX5 antibody -
Description
Rabbit polyclonal to IRX5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human IRX5 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab114000) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates: HEK293; HeLa; PC3; DU145.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatHorseCowDogZebrafishMacaque monkeyApplication Abreviews Notes WB 1/250. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Notes WB
1/250. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Target
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Function
Establishes the cardiac repolarization gradient by its repressive actions on the KCND2 potassium-channel gene. Required for retinal cone bipolar cell differentiation. May regulate contrast adaptation in the retina and control specific aspects of visual function in circuits of the mammalian retina (By similarity). Could be involved in the regulation of both the cell cycle and apoptosis in prostate cancer cells. -
Sequence similarities
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10265 Human
- Entrez Gene: 54352 Mouse
- Entrez Gene: 498918 Rat
- Omim: 606195 Human
- SwissProt: A2RRB5 Human
- SwissProt: P78411 Human
- SwissProt: Q9JKQ4 Mouse
- Unigene: 435730 Human
see all -
Alternative names
- Homeodomain protein IRX-2A antibody
- Homeodomain protein IRXB2 antibody
- Iroquois homeobox protein 5 antibody
see all
Images
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Western blot - Anti-IRX5 antibody (ab100939)All lanes : Anti-IRX5 antibody (ab100939) at 1/250 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
References (0)
ab100939 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-IRX5 antibody (ab100939)All lanes : Anti-IRX5 antibody (ab100939) at 1/250 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
