Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IRX4 antibody (ab123542)

Key features and details

  • Rabbit polyclonal to IRX4
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-IRX4 antibody

  • Description

    Rabbit polyclonal to IRX4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Macaque monkey, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human IRX4 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab139538)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab123542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

Images

  • Western blot - Anti-IRX4 antibody (ab123542)
    Western blot - Anti-IRX4 antibody (ab123542)
    Anti-IRX4 antibody (ab123542) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 20 minutes

References (2)

Publishing research using ab123542? Please let us know so that we can cite the reference in this datasheet.

ab123542 has been referenced in 2 publications.

  • Jia Z  et al. 1,25-dihydroxyvitamin D3 signaling-induced decreases in IRX4 inhibits NANOG-mediated cancer stem-like properties and gefitinib resistance in NSCLC cells. Cell Death Dis 11:670 (2020). PubMed: 32820157
  • Ward MC & Gilad Y A generally conserved response to hypoxia in iPSC-derived cardiomyocytes from humans and chimpanzees. Elife 8:N/A (2019). PubMed: 30958265

Images

  • Western blot - Anti-IRX4 antibody (ab123542)
    Western blot - Anti-IRX4 antibody (ab123542)
    Anti-IRX4 antibody (ab123542) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 20 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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