Anti-IRS2 antibody [EPR1650(2)] (ab133276)
![Anti-IRS2 antibody [EPR1650(2)] (ab133276)](https://www.abcam.com/ps/products/133/ab133276/Images/ab133276-415048-anti-irs2-antibody-epr16502-western-blot-hek293-wt-hek293-irs2-knockout-a375-a549.jpg "Anti-IRS2 antibody [EPR1650(2)] (ab133276)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1650(2)] to IRS2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-IRS2 antibody [EPR1650(2)]
See all IRS2 primary antibodies -
Description
Rabbit monoclonal [EPR1650(2)] to IRS2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human IRS2 aa 1250-1350. The exact sequence is proprietary.
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Positive control
- WB: A375, A549, 293T and 293T+insulin cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1650(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRS2 antibody [EPR1650(2)] (ab133276)All lanes : Anti-IRS2 antibody [EPR1650(2)] (ab133276) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : IRS2 knockout HEK-293 cell lysate
Lane 3 : A-375 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 137 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab133276 observed at 160 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab133276 was shown to react with IRS2 in wild-type HEK-293 cells in western blot with loss of signal observed in IRS2 knockout sample. Wild-type and IRS2 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab133276 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-IRS2 antibody [EPR1650(2)] (ab133276)](https://www.abcam.com/ps/products/133/ab133276/Images/ab133276-146741-anti-irs2-antibody-epr16502-western-blot.jpg)
Western blot - Anti-IRS2 antibody [EPR1650(2)] (ab133276)All lanes : Anti-IRS2 antibody [EPR1650(2)] (ab133276) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : 293T+insulin cell lysate
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 137 kDa
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![Anti-IRS2 antibody [EPR1650(2)] (ab133276)](https://www.abcam.com/ps/products/133/ab133276/Images/ab133276-3-benefits-of-recombinant-antibodies.png)
Anti-IRS2 antibody [EPR1650(2)] (ab133276)
