Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20441] to IRF8 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-IRF8 antibody [EPR20441] - BSA and Azide free
    See all IRF8 primary antibodies

  • Description

    Rabbit monoclonal [EPR20441] to IRF8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251475 is the carrier-free version of ab207418. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251475 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    This data was developed using ab207418, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IRF8 with ab207418 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human tonsil (×200) [PMID: 18663414] [PMID: 16380510]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    This data was developed using ab207418, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human non-hodgkin’s lymphoma tissue labeling IRF8 with ab207418 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human non-hodgkin’s lymphoma. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    Western blot - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    All lanes : Anti-IRF8 antibody [EPR20441] (ab207418) at 1/1000 dilution

    Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 1 minute


    This data was developed using ab207418, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    Western blot - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    All lanes : Anti-IRF8 antibody [EPR20441] (ab207418) at 1/1000 dilution

    Lane 1 : Human fetal spleen lysate
    Lane 2 : Human tonsil lysate
    Lane 3 : Human colon lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    This data was developed using ab207418, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 30 seconds; Lane 2: 2 seconds; Lane 3: 15 seconds.

  • Immunoprecipitation - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    Immunoprecipitation - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    This data was developed using ab207418, the same antibody clone in a different buffer formulation.IRF8 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab207418 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207418 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: Raji whole cell lysate 10 μg (Input). Lane 2: ab207418 IP in Raji whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207418 in Raji whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
  • Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
    Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.