Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)
![Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)](https://www.abcam.com/ps/products/251/ab251475/Images/ab251475-5-anti-irf8-antibody-epr20441-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20441] to IRF8 - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-IRF8 antibody [EPR20441] - BSA and Azide free
See all IRF8 primary antibodies -
Description
Rabbit monoclonal [EPR20441] to IRF8 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251475 is the carrier-free version of ab207418. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251475 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20441 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)This data was developed using ab207418, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IRF8 with ab207418 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human tonsil (×200) [PMID: 18663414] [PMID: 16380510]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)](https://www.abcam.com/ps/products/251/ab251475/Images/ab251475-4-anti-irf8-antibody-epr20441-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)This data was developed using ab207418, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human non-hodgkin’s lymphoma tissue labeling IRF8 with ab207418 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human non-hodgkin’s lymphoma. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Western blot - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)](https://www.abcam.com/ps/products/251/ab251475/Images/ab251475-406134-anti-irf8-antibody-epr20441-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)All lanes : Anti-IRF8 antibody [EPR20441] (ab207418) at 1/1000 dilution
Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 1 minuteThis data was developed using ab207418, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)](https://www.abcam.com/ps/products/251/ab251475/Images/ab251475-403415-anti-irf8-antibody-epr20441-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)All lanes : Anti-IRF8 antibody [EPR20441] (ab207418) at 1/1000 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human colon lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDaThis data was developed using ab207418, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 30 seconds; Lane 2: 2 seconds; Lane 3: 15 seconds.
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![Immunoprecipitation - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)](https://www.abcam.com/ps/products/251/ab251475/Images/ab251475-1-anti-irf8-antibody-epr20441-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)This data was developed using ab207418, the same antibody clone in a different buffer formulation.IRF8 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab207418 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207418 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: Raji whole cell lysate 10 μg (Input). Lane 2: ab207418 IP in Raji whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207418 in Raji whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second. -
![Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)](https://www.abcam.com/ps/products/251/ab251475/Images/ab251475-6-benefits-of-recombinant-antibodies.png)
Anti-IRF8 antibody [EPR20441] - BSA and Azide free (ab251475)