Anti-IQGAP3 antibody (ab88353)
Key features and details
- Mouse polyclonal to IQGAP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IQGAP3 antibody
See all IQGAP3 primary antibodies -
Description
Mouse polyclonal to IQGAP3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length Human protein (NP_839943.2)
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Positive control
- Placenta
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IQGAP3 antibody (ab88353)Anti-IQGAP3 antibody (ab88353) at 1 µg/ml + Human placenta lysate at 25 µg
Secondary
Goat anti-Mouse IgG at 1/5000 dilution
Predicted band size: 185 kDa
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Western blot - Anti-IQGAP3 antibody (ab88353)All lanes : Anti-IQGAP3 antibody (ab88353) at 1 µg/ml
Lane 1 : IQGAP3-transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG at 1/5000 dilution
Predicted band size: 185 kDa
Observed band size: 179 kDa why is the actual band size different from the predicted?
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Western blot - Anti-IQGAP3 antibody (ab88353) This image is courtesy of an anonymous AbreviewAll lanes : Anti-IQGAP3 antibody (ab88353) at 1/500 dilution
Lanes 1-2 : Mouse lung whole tissue lysate
Lanes 3-4 : Mouse lung adenocarcinoma whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated rabbit anti-mouse IgG monoclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 185 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutes
