Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)
![Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)](https://www.abcam.com/ps/products/192/ab192264/Images/ab192264-230106-anti-interferon-alpha-6-antibody-epr16355-western-blot.jpg "Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16355] to Interferon alpha 6
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Interferon alpha 6 antibody [EPR16355]
See all Interferon alpha 6 primary antibodies -
Description
Rabbit monoclonal [EPR16355] to Interferon alpha 6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U937, human fetal liver and human fetal lung lysates: Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16355 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)All lanes : Anti-Interferon alpha 6 antibody [EPR16355] (ab192264) at 1/5000 dilution
Lane 1 : U937 cell lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDaBlocking/dilution buffer: 5% NFDM /TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)](https://www.abcam.com/ps/products/192/ab192264/Images/ab192264-230105-anti-interferon-alpha-6-antibody-epr16355-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Interferon alpha 6 with ab192264 at 1/50 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue).
The negative controls are as follows;
1. Anti-Interferon alpha 6 at 1/50 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.
2. Anti-Interferon alpha 6 at 1/50 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution. -
![Flow Cytometry - Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)](https://www.abcam.com/ps/products/192/ab192264/Images/ab192264-262064-anti-interferon-alpha-6-antibody-epr16355-flow-cytometry.jpg)
Flow Cytometry - Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)Flow cytometry analysis of Jurkat cells labelling Interferon alpha 6 (red) with purified ab192264 at dilution of 1/600. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
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![Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)](https://www.abcam.com/ps/products/192/ab192264/Images/ab192264-4-benefits-of-recombinant-antibodies.png)
Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)
